Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Microbial lipase is one of the major sources enzyme that has been broadly exploited in food, detergent, and pharmaceutical industries due to its high catalytic activity, yield, environmental friendliness cost-effectiveness. Therefore, aim this study was optimize medium for submerged fermentation production by a novel strain, Bacillus salmalaya strain 139SI. The media subjected Luria Bertani (LB) with different carbon surfactants supplemented determine which would give highest activity salmalaya. Lipase supernatant containing ddetermined using titrimetric method hydrolysis reaction. Results showed olive oil used as source, induced (11.0 U/mL) compared sunflower (9.6 cooking waste (7.8 U/mL). For surfactants, LB tween 80 enhanced higher (6.8 20 (6.0 sodium dodecyl sulphate (SDS) (2.0 Thus, it can be concluded allows optimization culture whereby, among sources, production, whereas Tween best inducer other surfactants.